Modulation of rainbow trout (Oncorhynchus mykiss) intestinal immune gene expression following bacterial challenge.
نویسندگان
چکیده
The mucosal immune systems of fishes are still poorly understood, and defined models for studying natural host-pathogen interactions are lacking. The objective of this study was to evaluate different challenge models and pathogens to examine the magnitude of change in intestinal immune gene expression. Rainbow trout were exposed by immersion to Yersinia ruckeri or by intraperitoneal injection with Flavobacterium psychrophilum. At 3, 9, or 10 days post-challenge, pathogen load was quantified by plate count and intestinal tissue was removed and immune gene expression measured by real-time PCR. In general, the magnitude of infection was correlated with change in immune gene transcript abundance. We found that messages for the innate immune molecules, SAA, IL-8, INF-γ and TNF-α, as well as the message for IgM, were up-regulated in intestinal tissue in both challenge paradigms. A >250-fold increase was observed in SAA and 20-fold increase of IL-8 gene transcript abundance occurred on day 10 following challenge with F. psychrophilum. Within individual fish, there was a positive correlation between bacteria load in the spleen and the increase of immune gene message between 3 and 10 days post-infection. These findings demonstrate that measurable changes in immune gene expression occur in the intestine of rainbow trout following bath challenge with Y. ruckeri or injection challenge with F. psychrophilum.
منابع مشابه
Beneficial effects of a sodium butyrate source on growth performance, intestinal bacterial communities, digestive enzymes, immune responses and disease resistance in rainbow trout (Oncorhynchus mykiss)
The aim of the present study was to investigate the effects of dietary sodium butyrate on growth performance, intestinal microbiota, digestive enzymes, humoral immune responses and resistance against Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss) juveniles (39.5±1.12 g). The fish were fed diets supplemented with 0 (SB0), 1.5 (SB1.5), 2.5 (SB2.5) and 5 (SB5.0) g/kg sodium butyrate f...
متن کاملDataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay
A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e...
متن کاملEffect of chitosan oral consumption on intestinal bacterial flora of rainbow trout (Oncorhynchus mykiss)
Rainbow trout is one of the most important breeding species in the world. The use of growth promoters, immune stimulants and prebiotics can improve the breeding efficiency of this fish. This study seeks to determine the prebiotic effects of chitosan in salmon. For this purpose, chitosan (1%, 0.5% and 0.25%) was added to the diet of rainbow trout with an average weight of 180g. After 150 days, t...
متن کاملCharacterization of serum and mucosal antibody responses and relative per cent survival in rainbow trout, Oncorhynchus mykiss (Walbaum), following immunization and challenge with Flavobacterium psychrophilum
Serum and mucosal antibody responses of juvenile rainbow trout, Oncorhynchus mykiss, were characterized by enzyme-linked immunosorbent assay (ELISA) following immunization with various preparations of formalin-killed Flavobacterium psychrophilum cells. The protective nature of these preparations was then determined by immunizing rainbow trout fry and challenging with the bacterium. Juvenile rai...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Veterinary immunology and immunopathology
دوره 146 1 شماره
صفحات -
تاریخ انتشار 2012